1. Explain why antibodies allow scientists to aim and place specific disease agents. The primary antibodies attach to the antigens that have the same receptors and the secondary antibodies do the same with the primary antibodies.
2. Why is the secondary antibody used in an ELISA trial conjugated with an enzyme? What happens when this enzyme meets up with its substrate? The enzyme represents the arm that the secondary antibodies ( constabulary ) utilize to kill the antigen ( foreign object ) .
3. What does it intend if a disease can be detected in samples from one individual at a dilution of 1/5 and in another patient at a dilution of 1/100?
4. Describe a state of affairs that illustrates why it is a good thought to finish the ELISA check in triplicate.
5. Why do you believe college pupils populating in residence halls are frequently populations who see meningitis eruptions? Most pupils fail to clean decently their residence hall suites and the same goes to their anterior residence hall users. This consequences an eruption of bacteriums. viruses. parasites. etc. to distribute throughout the campus
6. How did ELISA informations allow you to track the way of infection at the college? With the blood sample given from Sue. scientists were able to place what type of bacteria/virus Sue contains and to find whether the infection is infective or non.
7. Discourse the restrictions of utilizing antigen concentration to infer the way of infection. Be certain to mention to the workings of the human immune system.
8. The ELISA trial can besides be used to observe antibodies that are produced in response to a specific antigen. Using information about how you completed this ELISA experiment. sketch a process for proving for antibodies in the blood.
9. Explain why in sudden eruptions. it may be better to prove for disease antigens instead than for antibodies. The antibodies are at that place to assist and kill of the antigens. but don’t contain the whole Deoxyribonucleic acid strand. The antigens have the specific DNA strand the scientists are seeking for to find the bacteria/virus.
10. Home gestation trials utilize ELISA engineering.
When a adult female is pregnant. her organic structure produces a endocrine call human chorionic gonadotrophin ( human chorionic gonadotropin ) . Explain how antibodies can be used to observe this endocrine and are linked to a colour alteration a adult female may see on a positive trial. The gestation trial traps the antigens that are attached to primary and secondary antibodies to the enzyme.